CED-10/Rac1 regulates endocytic recycling through the RAB-5 GAP TBC-2.

CED-10/Rac1 regulates endocytic recycling through the RAB-5 GAP TBC-2.

Blog Article

Rac1 is a founding member of the Rho-GTPase family and a key regulator of membrane remodeling.In the context of apoptotic cell corpse engulfment, CED-10/Rac1 acts with its bipartite guanine nucleotide exchange factor, CED-5/Dock180-CED-12/ELMO, in an evolutionarily conserved pathway to promote phagocytosis.Here we show Shirt that in the context of the Caenorhabditis elegans intestinal epithelium CED-10/Rac1, CED-5/Dock180, and CED-12/ELMO promote basolateral recycling.

Furthermore, we show that CED-10 binds to the RAB-5 GTPase activating protein TBC-2, that CED-10 contributes to recruitment of TBC-2 to endosomes, and that recycling cargo is trapped in recycling endosomes in ced-12, ced-10, and tbc-2 mutants.Expression of GTPase defective RAB-5(Q78L) also traps recycling cargo.Our results indicate that down-regulation of early endosome regulator RAB-5/Rab5 by a tenga flip orb CED-5, CED-12, CED-10, TBC-2 cascade is an important step in the transport of cargo through the basolateral recycling endosome for delivery to the plasma membrane.